Microbiology

About the Department

Microbiology department is located in the first floor of the college building consisting of two wings. One of the wings has administrative section, demonstration hall, media preparation room, washing and sterilization room, and sections for mycology, mycobacteriology, parasitology, anaerobic bacteriology and rooms for faculty members. The other wing consists of student’s practical laboratory, library cum seminar room, research laboratory for postgraduates and faculty members. Apart from this, the department is having a separate diagnostic division in Hospital Central Laboratory where routine clinical diagnostic work is carried out.There is a lecture hall allotted to the department, which can hold about 200 students. The area of the lecture hall is approximately 1600 sq.ft. Audio visuals are available for teaching. The animal house is under the control of Microbiology.

Academics

Involved in Teaching for

II MBBS

II BDS

BPT III Sem

BSc Nursing : Regular

Diploma in Nursing

Basic Sciences for postgraduates

Facilities / Equipments

In this department speciments cullected and pursuit of excellence in reporting, we participate in a national external quality control programme conducted by Christian Medical College , Vellore and over the last 3 years, our laboratory has been rated very high.

Diagnostic Laboratory:

Hospital Central laboratory is situated in the ground floor of the Hospital. The investigations carried out in the department of Microbiology are as follows:

  • Clinical BacteriologyThe following specimens are received from outpatients and inpatients of the hospital for culture.Blood culture:
    •   – Versatrek culture system has been installed.
    •   – Respiratory – Throat Swab
    •   – Exudates like Urine – semiquantitative method is done; Faeces – Swabs from different sites
    •   – Organisms are identified by carventional biochemical and other tests and antibiotic sensitivity testing is done by disc diffusion method.
  • Serological tests:
    •   – Widal – For enteric fever. Take agglutination test. Commercial kits are used
    •   – RPR – Syphilis;TPHA is done under special circumstances
    •   – ASO Tibe, CRP detection, RF detection are carried out by Latex agglutination test
    •   – ELISA (Torch KIT) is carried out for Toxoplasma, Rubella, Cytomegalocirus, Herpes Infection
    •   – Leptospirosis – ELISA IgM
    •   – Dengue – Rapid ELISA
  • Mycology:
    •   – KOH mount for fungal elements
    •   – India ink preparation for cryptococcus.
    •   – Fungal Culture for  Conventional Versatresk
    •   – B.O.D incubator
  • Mycobacteriology:
    •   – Direct smear for Acid fast bacilli. L.J Medium for Conventional culture;Versatrk – Under trial
  • Parasitology:
    •   – Faeces examination for ova or cyst is done by routine Saline and iodine preparation. Modified acid fast staining – for cryptosporidium.
  • Theatre surveillence is done for all the operation theatres once in a month:
    •   – Settle plate method
    •   – Culture of swabs
  • Water Bacteriology:
    •   – Water samples from different sites in the Hospital and Hosptel campus are periodically checked for faecal pollution.
  • Special Microscopy:
    •   – Dark ground microscopy is done for visualizing Leptospites in the clinical specimens and report is give in correlation with serology
  • Fluorescent Microscopy:
    •   – The staining procedures are being standardized
  • Virology and PCR sections:
    •   – Preparations are being made to establish the virology and PCR sections.

Faculty Details