CME & Workshop | Basic Principles and Trouble Shooting in Immunohistochemistry

The CME on 24th started with a lecture on Basic principles of IHC by Mr.Venugopal Assistant manager Dako (DSS).The Defination, Basic Protocol, and the importance of pre analytical, analytical  and post analytical variables and trouble shooting was explained in detail. The errors that could be committed and how to approach and correct them were also mentioned.

This was followed by FISH technique basic and updated by Mr.Arivazhagan, and the equipment required for FISH set up in the Lab and the use and maintenance of each equipment was covered.

The Inaugural ceremony was well attended by PG and faculty from different departments Surgical Gastroenterology, General Medicine, General Surgery, Orthopedics and Pre and Para department of Biochemistry Anatomy Physiology, Pharmacology and Microbiology.

The Chief Guest Dr.Sundaravalli devi could not attend the inaugural function through zoom because of prior engagement. The Dean Dr.S.P.Rao , Medical Superintendent Dr.Hariprasad reddy, Academic Director Dr.Narendra reddy lighted the lamp .

Post inaugural session started with a talk by Dr.Janaki- Professor& HOD Nandyala Medical college Kurnool.She was quite elaborate and described the clinical, gross, Light Microscopy, IHC, and  molecular findings of endometrial  hyperplasia vs endometrial carcinoma. It was well appreciated by all the students.

This session was followed by ICC/IHC of lung lesions by Dr.K.Durga Professor& HOD department of Pathology Narayana medical college and hospital Nellore. In the talk  both IHC on cytological smears and tissues was explained. The application of both diagnostic and predictive biomarkers  on smears/small biopsies of the lung was mentioned in detail keeping in mind the administration of targeted therapy for lung carcinomas.

Post  lunch session was by  Dr.Ramaswamy who spoke on the topic of soft tissue tumours and   covered both the routine as well as the IHC markers of soft tissue. The daunting task of Soft tissue tumours was  handled well by Dr Ramaswamy who made the subject realively easy for the post graduates.

Dr Vydehi”s talk on the 2021 classification of CNS tumours simplified the formidable topic .She was insistent on unlearning  what was already learnt and relearning the new classification in view of the vast number of changes inclusive of molecular genetics in the neoplasms.

The topic of Upgraded Equipment for automated IHC staining was shown by Mr Venugopal in pictorial mode.

The first day ended with an exciting and  gripping Quiz by Dr Durga with enthusiastic participation by the student delegates.

The 2nd day was the hands on workshop where the protocol of IHC was shown and explained in detail by Mr Venugopal .He took the students in a step by step manner through the entire sequence of staining and each of the 50 students inclusive of 6 NMCH technicians handled the staining themselves and the final stained product was amazing giving them complete satisfaction.

The workshop was followed by Valedictory function with review and recap by senior Professor Dr Sujata and Dr Vydehi with feedback and comments by delegates from other institutes.

Dr Karri  Maruti, Observor Dr NTRUHS gave her honest opinion about the CME and Workshop. She appreciated the organising skills and team spirit of the Department, the topics covered and the brilliant presentations of the speakers.

The Quiz prizes were awarded as below.

First Prize –        Dr A.T Gayatri  SVMC Tirupati.

Second Prize –        Dr N. Bhavana SVMC Tirupati

Third prize was shared by –    Dr Silana –  Narayana Medical College Hospital,  – Dr Chandana- Shantiram Medical College, Nandyala.